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Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping

United States Patent

9,285,575
March 15, 2016
View the Complete Patent at the US Patent & Trademark Office
A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-linear interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.
Xie; Xiaoliang Sunney (Lexington, MA), Freudiger; Christian (Boston, MA), Min; Wei (Cambridge, MA)
PRESIDENT AND FELLOWS OF HARVARD COLLEGE (Cambridge, MA)
12/ 690,579
20100188496
January 20, 2010
This invention was made with government support under DE-FG02-07ER15875 awarded by the U.S. Department of Energy, OD000277 awarded by the National Institutes of Health, and 0649892 awarded by the National Science Foundation. The government has certain rights to the invention.