Skip to Content
Find More Like This
Return to Search

RNA-guided transcriptional regulation

United States Patent

9,267,135
February 23, 2016
View the Complete Patent at the US Patent & Trademark Office
Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.
Church; George M. (Brookline, MA), Mali; Prashant G. (Somerville, MA), Esvelt; Kevin M. (Cambridge, MA)
President and Fellows of Harvard College (Cambridge, MA)
14/ 319,289
20140356959
June 30, 2014
STATEMENT OF GOVERNMENT INTERESTS This invention was made with government support under Grant No. P50 HG005550 from the National Institutes of health and DE-FG02-02ER63445 from the Department of Energy. The government has certain rights in the invention.