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Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression

United States Patent

December 8, 2015
View the Complete Patent at the US Patent & Trademark Office
Pacific Northwest National Laboratory - Visit the Technology Commercialization Program Website
Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
Dai; Ziyu (Richland, WA), Baker; Scott E. (Richland, WA)
Battelle Memorial Institute (Richland, WA)
14/ 703,499
May 4, 2015
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT This invention was made with government support under contract number DE-AC05-76RLO1830 awarded by the U.S. Department of Energy. The government has certain rights in the invention.