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Multifunctional cellulase and hemicellulase

United States Patent

September 29, 2015
View the Complete Patent at the US Patent & Trademark Office
A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.
Fox; Brian G. (Madison, WI), Takasuka; Taichi (Madison, WI), Bianchetti; Christopher M. (Madison, WI)
Wisconsin Alumni Research Foundation (Madison, WI)
14/ 030,290
September 18, 2013
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH This invention was made with government support under DE-FC02-07ER64494 awarded by the US Department of Energy and GM094584 awarded by the National Institutes of Health. The government has certain rights in the invention.