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Genes encoding chavicol/eugenol synthase from the creosote bush Larrea tridentata

United States Patent

September 15, 2015
View the Complete Patent at the US Patent & Trademark Office
Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant ro culture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
Lewis; Norman G. (Pullman, WA), Davin; Laurence B. (Pullman, WA), Kim; Sung-Jin (Pullman, WA), Vassao; Daniel Giddings (Pullman, WA), Patten; Ann M. (Pullman, WA), Eichinger; Dietmar (Schwetzingen, DE)
Washington State University (Pullman, WA)
12/ 307,343
May 29, 2007
STATEMENT REGARDING FEDERAL SUPPORT Particular aspects of this invention were supported by National Science Foundation Grants MCB 04-1729 and Department of Energy Grant DE-FG-0397ER20259, National Institute of General Medical Sciences grant 5-R01-GM-066173-02, NSF grant MCB-0417291, and the United States Government has certain rights.