Skip to Content
Find More Like This
Return to Search

Organic acid-tolerant microorganisms and uses thereof for producing organic acids

United States Patent

May 6, 2014
View the Complete Patent at the US Patent & Trademark Office
Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.
Pfleger; Brian Frederick (Madison, WI), Begemann; Matthew Brett (Madison, WI)
Wisconsin Alumni Research Foundation (Madison, WI)
13/ 798,835
March 13, 2013
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH This invention was made with government support under FA9550-11-1-0038 awarded by the USAF/AFOSR and DE-FC02-07ER64494 awarded by the US Department of Energy. The government has certain rights in the invention.