Chimeric proteins comprising a sequence nonspecific single-stranded nucleic-acid-binding domain joined to a catalytic nucleic-acid-modifying domain are provided. Methods comprising contacting a nucleic acid molecule with a chimeric protein, as well as systems comprising a nucleic acid molecule, a chimeric protein, and an aqueous solution are also provided. The joining of sequence nonspecific single-stranded nucleic-acid-binding domain and a catalytic nucleic-acid-modifying domain in chimeric proteins, among other things, may prevent the separation of the two domains due to their weak association and thereby enhances processivity while maintaining fidelity.
STATEMENT OF GOVERNMENT INTEREST
This disclosure was made with support under Grant Number RSG-03-051-01-GMC awarded by the American Cancer Society and Contract No. W-31-109-ENG-38 awarded by the U.S. Department of Energy, Office of Energy Research. The U.S. government has certain rights in the invention.