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High processivity polymerases

United States Patent

June 10, 2014
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
Chimeric proteins comprising a sequence nonspecific single-stranded nucleic-acid-binding domain joined to a catalytic nucleic-acid-modifying domain are provided. Methods comprising contacting a nucleic acid molecule with a chimeric protein, as well as systems comprising a nucleic acid molecule, a chimeric protein, and an aqueous solution are also provided. The joining of sequence nonspecific single-stranded nucleic-acid-binding domain and a catalytic nucleic-acid-modifying domain in chimeric proteins, among other things, may prevent the separation of the two domains due to their weak association and thereby enhances processivity while maintaining fidelity.
Shamoo; Yousif (Houston, TX), Sun; Siyang (Lafayette, IN)
William Marsh Rice University (Houston, TX)
11/ 945,877
November 27, 2007
STATEMENT OF GOVERNMENT INTEREST This disclosure was made with support under Grant Number RSG-03-051-01-GMC awarded by the American Cancer Society and Contract No. W-31-109-ENG-38 awarded by the U.S. Department of Energy, Office of Energy Research. The U.S. government has certain rights in the invention.