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Buoyant triacylglycerol-filled green algae and methods therefor

United States Patent

April 14, 2015
View the Complete Patent at the US Patent & Trademark Office
Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.
Goodenough; Ursula (St. Louis, MO), Goodson; Carrie (St. Louis, MO)
Washington University (Saint Louis, MO)
13/ 662,549
October 29, 2012
GOVERNMENT SUPPORT This work received support from a grant to the NAABB Consortium from the U.S. Department of Energy. The government may have certain rights in the invention.