Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).
STATEMENT OF GOVERNMENTAL SUPPORT
This invention was made in part by the US DOE Office of Biological and Environmental Research Genomic Sciences research program and the LLNL Laboratory Directed Research and Development (LDRD) program with government support under Contract No. DE-AC02-05CH11231 and under Contract DE-AC52-07NA27344 awarded by the U.S. Department of Energy. The government has certain rights in the invention.