Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IBB 303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.
STATEMENT OF GOVERNMENT RIGHTS
This invention was made with government support under Contract No. DE-ACO2-05CH11231 awarded by the U.S. Department of Energy and through DOE/NNSA GIPP program CRADA with Verenium Corp. (formerly Diversa Corp.); Subcontract # CRADA (BG0301700) STCU P196. The government has certain rights in the invention.