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Hydrogel nanoparticle based immunoassay

United States Patent

April 21, 2015
View the Complete Patent at the US Patent & Trademark Office
An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.
Liotta; Lance A. (Bethesda, MD), Luchini; Alessandra (Fairfax, VA), Petricoin; Emanuel F. (Gainesville, VA), Espina; Virginia (Rockville, MD)
George Mason Research Foundation, Inc. (Fairfax, VA)
13/ 061,507
August 26, 2009
GOVERNMENT LICENSE RIGHTS This invention was made with government support under DE-FC52-04NA25455, awarded by the Department of Energy. The government has certain rights in the invention.