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Restriction/modification polypeptides, polynucleotides, and methods

United States Patent

February 24, 2015
View the Complete Patent at the US Patent & Trademark Office
Oak Ridge National Laboratory - Visit the Partnerships Directorate Website
The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.
Westpheling; Janet (Bogart, GA), Chung; DaeHwan (Athens, GA), Huddleston; Jennifer (Abilene, TX), Farkas; Joel A. (Athens, GA)
University of Georgia Research Foundation, Inc. (Athens, GA)
13/ 439,069
April 4, 2012
GOVERNMENT FUNDING The present invention was made with government support under Grant No. DE-AC05-000R22725, awarded by the U.S. Department of Energy, BioEnergy Science Center. The Government has certain rights in this invention.