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Methods, microfluidic devices, and systems for detection of an active enzymatic agent

United States Patent

8,871,496
October 28, 2014
View the Complete Patent at the US Patent & Trademark Office
Sandia National Laboratories - Visit the Intellectual Property Management and Licensing Website
Microfluidic Devices
Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.
Sommer; Gregory J. (Livermore, CA), Hatch; Anson V. (Tracy, CA), Singh; Anup K. (Danville, CA), Wang; Ying-Chih (Pleasanton, CA)
Sandia Corporation (Albuquerque, NM)
12/ 544,982
August 20, 2009
STATEMENT REGARDING RESEARCH & DEVELOPMENT The United States Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of contract No. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation.