Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH
This invention was made with government support under FA9550-11-1-0038 awarded by the USAF/AFOSR, DE-FC02-07ER64494 awarded by the US Department of Energy, and 1240268 awarded by the National Science Foundation. The government has certain rights in the invention.