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Selective posttranslational modification of phage-displayed polypeptides

United States Patent

November 19, 2013
View the Complete Patent at the US Patent & Trademark Office
The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2] cycloaddition reactions and Staudinger modifications.
Tsao; Meng-Lin (San Diego, CA), Tian; Feng (San Diego, CA), Schultz; Peter (La Jolla, CA)
The Scripps Research Institute (La Jolla, CA)
11/ 580,223
October 11, 2006
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT This invention was made with government support from the Department of Energy under Grant No. ER46051, and the National Institutes of Health under Grant No. GM56528. The government may have certain rights to this invention.