Reagents and methods are provided that permit simultaneous analysis of multiple diverse small molecule analytes present in a complex mixture. Samples are labeled with chemically identical but isotopically distinct forms of the labeling reagent, and analyzed using mass spectrometry. A single reagent simultaneously derivatizes multiple small molecule analytes having different reactive functional groups.
This invention was made with government support under Grant No. R33DK070290, awarded by the National Institutes of Health, Grant No. DE-FC36-07GO17059, awarded by the U.S. Department of Energy, and Grant No. DBI0421102, awarded by the National Science Foundation. The Government has certain rights in the invention.