Skip to Content
Find More Like This
Return to Search

Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

United States Patent

8,470,537
June 25, 2013
View the Complete Patent at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.
Gardner; Shea N. (San Leandro, CA), Mariella, Jr.; Raymond P. (Danville, CA), Christian; Allen T. (Tracy, CA), Young; Jennifer A. (Berkeley, CA), Clague; David S. (Livermore, CA)
Lawrence Livermore National Security, LLC (Livermore, CA)
12/ 959,789
20110104763
December 3, 2010
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT The United States Government has rights in this invention pursuant to Contract No. DE-AC52-07NA27344 between the United States Department of Energy and Lawrence Livermore National Security, LLC for the operation of Lawrence Livermore National Laboratory.