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Expression of eukaryotic polypeptides in chloroplasts

United States Patent

June 4, 2013
View the Complete Patent at the US Patent & Trademark Office
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
Mayfield; Stephen P. (Cardiff, CA)
The Scripps Research Institute (La Jolla, CA)
11/ 197,730
January 16, 1998
This invention was made with government support under Contract No. GM 54659 by the National Institutes of Health and Contract No. DO-FG03-93ER20116 by the U.S. Department of Energy. The government has certain rights in the invention.