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Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins

United States Patent

June 4, 2013
View the Complete Patent at the US Patent & Trademark Office
Synthesizing Membrane Proteins Using In Vitro Methodology (ANL-IN-06-099)
The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes.
Laible; Philip D. (Villa Park, IL), Hanson; Deborah K. (Villa Park, IL)
Uchicago Argonne, LLC (Chicago, IL)
12/ 702,167
February 8, 2010
CONTRACTUAL ORIGIN OF THE INVENTION The U.S. Government has rights in this invention pursuant to Contract No. AC02-06CH11357 between the U.S. Department of Energy and UChicago Argonne, LLC.