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Method for in vitro recombination

United States Patent

May 7, 2013
View the Complete Patent at the US Patent & Trademark Office
The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.
Gibson; Daniel Glenn (Crofton, MD), Smith; Hamilton O. (San Diego, CA)
Synthetic Genomics, Inc. (La Jolla, CA)
12/ 820,861
June 22, 2010
STATEMENT OF RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH This invention was made with government support under Grant No. DE-FG02-02ER63453 awarded by the Department of Energy. The government has certain rights in the invention.