The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
The invention was made with U.S. Government support under Grant No. 0073590 awarded by the National Science Foundation, Grant No. F49620-01-1-0467 awarded by the Air Force Research Laboratory, and Grant No. DE-FG02-01ER45922 awarded by the Department of Energy. The Government has certain rights in this invention.