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Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme

United States Patent

January 29, 2013
View the Complete Patent at the US Patent & Trademark Office
U.S. Department of Energy - Visit the Office of the Assistant General Counsel for Technology Transfer & Intellectual Property Website
Cytochrome P450 CYP153A6 from Myobacterium sp. strain HXN1500 was engineered using in-vivo directed evolution to hydroxylate small-chain alkanes regioselectively. Mutant CYP153A6-BMO1 selectively hydroxylates butane and pentane at the terminal carbon to form 1-butanol and 1-pentanol, respectively, at rates greater than wild-type CYP153A6 enzymes. This biocatalyst is highly active for small-chain alkane substrates and the regioselectivity is retained in whole-cell biotransformations.
Koch; Daniel J. (Seeheim-Jugenheim, DE), Arnold; Frances H. (La Canada, CA)
U.S. Department of Energy (Washington, DC)
12/ 619,033
November 16, 2009
GOVERNMENT INTERESTS The United States Government has rights in this invention pursuant to Contract No. DE-FG02-06ER15762, between the U.S. Department of Energy (DOE) and the California Institute of Technology.