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Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture

United States Patent

8,338,166
December 25, 2012
View the Complete Patent at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
Beer; Neil R. (Pleasanton, CA), Hindson; Benjamin J. (Livermore, CA), Colson, Jr.; Billy W. (San Ramon, CA), Fitch; Joseph P. (Livermore, CA)
Lawrence Livermore National Security, LLC (Livermore, CA)
11/ 650,363
20080166793
January 4, 2007
The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.