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Regioselective alkane hydroxylation with a mutant AlkB enzyme

United States Patent

8,309,333
November 13, 2012
View the Complete Patent at the US Patent & Trademark Office
U.S. Department of Energy - Visit the Office of the Assistant General Counsel for Technology Transfer & Intellectual Property Website
AlkB from Pseudomonas putida was engineered using in-vivo directed evolution to hydroxylate small chain alkanes. Mutant AlkB-BMO1 hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. Mutant AlkB-BMO2 similarly hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. These biocatalysts are highly active for small chain alkane substrates and their regioselectivity is retained in whole-cell biotransformations.
Koch; Daniel J. (Seeheim-Jugenheim, DE), Arnold; Frances H. (La Canada, CA)
The United States of America, as represented by Department of Energy (Washington, DC)
12/ 618,960
November 16, 2009
GOVERNMENT INTERESTS The United States Government has rights in this invention pursuant to Contract No. DE-FG02-06ER15762, between the U.S. Department of Energy (DOE) and the California Institute of Technology.