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Method for preparation of single chain antibodies

United States Patent

April 3, 2012
View the Complete Patent at the US Patent & Trademark Office
This invention provides a method for identifying cells expressing a target single chain antibody (scFv) directed against a target antigen from a collection of cells that includes cells that do not express the target scFv, comprising the step of combining the collection of cells with an anti-idiotype directed to an antibody specific for the target antigen and detecting interaction, if any, of the anti-idiotype with the cells, wherein the occurrence of an interaction identifies the cell as one which expresses the target scFv. This invention also provides a method for making a single chain antibody (scFv) directed against an antigen, wherein the selection of clones is made based upon interaction of those clones with an appropriate anti-idiotype, and heretofore inaccessible scFv so made. This invention provides the above methods or any combination thereof. Finally, this invention provides various uses of these methods.
Cheung; Nai-Kong V. (New York, NY), Guo; Hong-fen (New York, NY)
Sloan-Kettering Institute for Cancer Research (New York, NY)
12/ 709,848
February 22, 2010
This application was supported in part by Department Of Energy Grant No. DE-FG-02-93ER61658, National Institute of Health Grant No. CA61017 and National Cancer Institute Grant No. NCICA 89936. Accordingly, the United States Government may have certain rights in this invention.