A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.
This invention was made with government support under Grant No. DE-FG03-01ER25499, awarded by the Department of Energy and Grant No. BES0120277, awarded by the National Science Foundation. The Government has certain rights in the invention.