An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.
STATEMENT OF GOVERNMENTAL SUPPORT
This invention was made during work supported by National Institutes of Health GM-62412 and U.S. Department of Energy under Contract No. DE-AC03-76SF00098, now DE-Ac02-05CH11231. The government has certain rights in this invention.