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High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins

United States Patent

January 31, 2012
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.
Kim; Sung-Hou (Moraga, CA), Kim; Rosalind (Moraga, CA), Jancarik; Jamila (Walnut Creek, CA)
The Regents of the University of California (Oakland, CA)
11/ 393,209
March 29, 2006
STATEMENT OF GOVERNMENTAL SUPPORT This invention was made during work supported by National Institutes of Health GM-62412 and U.S. Department of Energy under Contract No. DE-AC03-76SF00098, now DE-Ac02-05CH11231. The government has certain rights in this invention.