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Aptamers and methods for their in vitro selection and uses thereof

United States Patent

January 31, 2012
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.
Doyle; Sharon A. (Walnut Creek, CA), Murphy; Michael B. (Severna Park, MD)
The Regents of the University of California (Oakland, CA)
12/ 027,239
February 6, 2008
STATEMENT OF GOVERNMENT SUPPORT This invention was made during work supported by the U.S. Department of Energy at Lawrence Livermore National Laboratory under contract No. W-7405-ENG-48, Lawrence Berkeley National Laboratory under contract No. DE-AC03-76SF00098, and Los Alamos National Laboratory under contract No. W-7405-ENG-36. The government has certain rights in this invention.