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Engineered microorganisms capable of producing target compounds under anaerobic conditions

United States Patent

January 17, 2012
View the Complete Patent at the US Patent & Trademark Office
The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
Buelter; Thomas (Denver, CO), Meinhold; Peter (Denver, CO), Feldman; Reid M. Renny (San Francisco, CA), Hawkins; Andrew C. (Parker, CO), Urano; Jun (Irvine, CA), Bastian; Sabine (Pasadena, CA), Arnold; Frances (La Canada, CA)
Gevo, Inc. (Englewood, CO), California Institute of Technology (Pasadena, CA)
13/ 269,937
October 10, 2011
STATEMENT REGARDING FEDERALLY FUNDED RESEARCH This invention was made with government support under contract DE-FG02-07ER84893, awarded by the Department of Energy. The government has certain rights in the invention.