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High expression Zymomonas promoters

United States Patent

August 2, 2011
View the Complete Patent at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
Viitanen; Paul V. (West Chester, PA), Tao; Luan (Havertown, PA), Zhang; Yuying (New Hope, PA), Caimi; Perry G. (Kennett Square, PA), McCole; Laura (East Fallowfield, PA), Zhang; Min (Lakewood, CO), Chou; Yat-Chen (Lakewood, CO), McCutchen; Carol M. (Wilmington, DE), Franden; Mary Ann (Centennial, CO)
E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC (Wilmington, DE)
12/ 410,495
March 25, 2009
STATEMENT OF GOVERNMENT RIGHTS This invention was made with United States government support under Contract Nos. 04-03-CA-70224 and DE-FC36-03GO13146 awarded by the Department of Energy. The United States government has certain rights in this invention. Further, the United States Government has rights in this invention under Contract No. DE-AC36-99GO10337 between the United States Department of Energy and the National Renewable Energy Laboratory, a Division of the Midwest Research Institute.