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Methods for analyzing nucleic acid sequences

United States Patent

May 17, 2011
View the Complete Patent at the US Patent & Trademark Office
The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.
Korlach; Jonas (Ithaca, NY), Webb; Watt W. (Ithaca, NY), Levene; Michael (Ithaca, NY), Turner; Stephen (Ithaca, NY), Craighead; Harold G. (Ithaca, NY), Foquet; Mathieu (Ithaca, NY)
Cornell Research Foundation (Ithaca, NY)
11/ 336,122
January 19, 2006
This invention was made with funds provided by the U.S. Government under National Science Foundation Grant No. BIR8800278, National Institutes of Health Grant No. P412RR04224-11, and Department of Energy Grant No. 066898-0003891. The U.S. Government may have certain rights in this invention.