Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT
A portion of the work described herein was supported by Grant No. GM62159 from the National Institutes of Health, grant number N0001498F0402 from the Office of Naval Research, and grants DE-FG03-00ER45812 and DE-AC03-76SF00098 from the Department of Energy. The United States Government has certain rights in the invention.