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Hybridization-based biosensor containing hairpin probes and use thereof

United States Patent

October 12, 2010
View the Complete Patent at the US Patent & Trademark Office
A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.
Miller; Benjamin L. (Penfield, NY), Strohsahl; Christopher M. (Rochester, NY)
University of Rochester (Rochester, NY)
11/ 838,616
August 14, 2007
The present invention was made in part with funding by the Department of Energy under grant DE-FG-02-02ER63410.A000. The U.S. government has certain rights in this invention.