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Cysteine-containing peptide tag for site-specific conjugation of proteins

United States Patent

7,807,778
October 5, 2010
View the Complete Patent at the US Patent & Trademark Office
The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety bound to the targeting moiety; the biological conjugate having a covalent bond between the thiol group of SEQ ID NO:2 and a functional group in the binding moiety. The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety that comprises an adapter protein, the adapter protein having a thiol group; the biological conjugate having a disulfide bond between the thiol group of SEQ ID NO:2 and the thiol group of the adapter protein. The present invention is also directed to biological sequences employed in the above biological conjugates, as well as pharmaceutical preparations and methods using the above biological conjugates.
Backer; Marina V. (Simsbury, CT), Backer; Joseph M. (Simsbury, CT)
SibTech, Inc. (Brookfield, CT)
12/ 075,811
20080312410
March 14, 2008
STATEMENT OF GOVERNMENT SUPPORT This invention was made in part with government support under grant numbers 1R43 HL61143-01; 1-R43-CA113080-01; R43AI054060-01; and 1R43 GM072170-01 from the National Institutes of Health, and grant number DE-FG-02-02ER83520 from the Department of Energy. The government has certain rights in this invention.