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Methods for validating the presence of and characterizing proteins deposited onto an array

United States Patent

7,799,570
September 21, 2010
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
A method of determining if proteins have been transferred from liquid-phase protein fractions to an array comprising staining the array with a total protein stain and imaging the array, optionally comparing the staining with a standard curve generated by staining known amounts of a known protein on the same or a similar array; a method of characterizing proteins transferred from liquid-phase protein fractions to an array including staining the array with a post-translational modification-specific (PTM-specific) stain and imaging the array and, optionally, after staining the array with a PTM-specific stain and imaging the array, washing the array, re-staining the array with a total protein stain, imaging the array, and comparing the imaging with the PTM-specific stain with the imaging with the total protein stain; stained arrays; and images of stained arrays.
Schabacker; Daniel S. (Naperville, IL)
Uchicago Argonne, LLC (Chicago, IL)
11/ 693,320
20080241965
March 29, 2007
STATEMENT OF GOVERNMENT SUPPORT This invention was partially conceived under Contract No. W-31-109-ENG-38 between the U.S. Department of Energy and the University of Chicago representing Argonne National Laboratory. Therefore, the Government has certain rights in the invention.