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Replica amplification of nucleic acid arrays

United States Patent

August 31, 2010
View the Complete Patent at the US Patent & Trademark Office
Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.
Church; George M. (Brookline, MA), Mitra; Robi D. (Chestnut Hill, MA)
President and Fellows of Harvard College (Cambridge, MA)
11/ 745,806
May 8, 2007
STATEMENT OF GOVERNMENT INTERESTS This application was funded in part by Department of Energy Grant No. DEFG02-87ER-60565. The government has certain rights to the invention.