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Quantum dot conjugates in a sub-micrometer fluidic channel

United States Patent

April 13, 2010
View the Complete Patent at the US Patent & Trademark Office
A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.
Stavis; Samuel M. (Ithaca, NY), Edel; Joshua B. (Brookline, MA), Samiee; Kevan T. (Ithaca, NY), Craighead; Harold G. (Ithaca, NY)
Cornell Research Foundation, Inc. (Ithaca, NY)
12/ 143,334
June 20, 2008
GOVERNMENT FUNDING The invention described herein was made with U.S. Government support under Grant Number DE-FG02-99ER62809 awarded by the Department of Energy. The United States Government has certain rights in the invention.