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Convergent synthesis of proteins by kinetically controlled ligation

United States Patent

March 9, 2010
View the Complete Patent at the US Patent & Trademark Office
The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.
Kent; Stephen (Chicago, IL), Pentelute; Brad (Chicago, IL), Bang; Duhee (Boston, MA), Johnson; Erik (Chicago, IL), Durek; Thomas (Chicago, IL)
The University of Chicago (Chicago, IL)
11/ 545,923
October 10, 2006
This invention was made with government support under grant number DE-FG02-04ER63786 awarded by the Department of Energy and grant number DMR-0213745 awarded by the National Science Foundation MRSEC. The government has certain rights in the invention.