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Method for converting sucrose to .beta.-D-glucose

United States Patent

July 7, 2009
View the Complete Patent at the US Patent & Trademark Office
Sandia National Laboratories - Visit the Intellectual Property Management and Licensing Website
Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.
Simmons; Blake A. (San Francisco, CA), Volponi; Joanne V. (Livermore, CA), Ingersoll; David (Albuquerque, NM), Walker; Andrew (Woodinville, WA)
Sandia Corporation (Livermore, CA)
11/ 825,615
July 5, 2007
STATEMENT OF GOVERNMENT INTEREST This invention was made with Government support under government contract DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention, including a paid-up license and the right, in limited circumstances, to require the owner of any patent issuing in this invention to license others on reasonable terms.