Compositions and methods of producing components of protein biosynthetic machinery that include glutamyl orthogonal tRNAs, glutamyl orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of glutamyl tRNAs/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
This invention was made with U.S. Government support under Contract No. GM 62159 from the National Institutes of Health and Contract No. DE-FG03-00ER45812 from the Department of Energy. The U.S. Government has certain rights to the invention.