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System and method for detecting cells or components thereof

United States Patent

January 6, 2009
View the Complete Patent at the US Patent & Trademark Office
Ames Laboratory - Visit the Iowa State University Research Foundation - Office of Intellectual Property & Technology Transfer Website
A system and method for detecting a detectably labeled cell or component thereof in a sample comprising one or more cells or components thereof, at least one cell or component thereof of which is detectably labeled with at least two detectable labels. In one embodiment, the method comprises: (i) introducing the sample into one or more flow cells of a flow cytometer, (ii) irradiating the sample with one or more light sources that are absorbed by the at least two detectable labels, the absorption of which is to be detected, and (iii) detecting simultaneously the absorption of light by the at least two detectable labels on the detectably labeled cell or component thereof with an array of photomultiplier tubes, which are operably linked to two or more filters that selectively transmit detectable emissions from the at least two detectable labels.
Porter; Marc D. (Ames, IA), Lipert; Robert J. (Ames, IA), Doyle; Robert T. (Ames, IA), Grubisha; Desiree S. (Corona, CA), Rahman; Salma (Ames, IA)
Iowa State University Research Foundation, Inc. (Ames, IA)
11/ 095,098
March 31, 2005
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT This invention was supported in part by the U.S. Department of Energy, Grant No. W-7405-ENG-82, and the U.S. Department of Agriculture, Grant No. 2002-35201-12659. The Government may have certain rights in this invention.