Skip to Content
Find More Like This
Return to Search

Structural determination of intact proteins using mass spectrometry

United States Patent

May 6, 2008
View the Complete Patent at the US Patent & Trademark Office
Sandia National Laboratories - Visit the Intellectual Property Management and Licensing Website
The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments in the mass spectrometer, eliminating complex microseparations, and protein preparatory chemical steps necessary for cross-linking based protein structure determination. Additionally, the preferred method of the present invention involves the determination of protein structures utilizing a top-down analysis of protein structures to search for covalent modifications. In the preferred method, intact proteins are ionized and fragmented within the mass spectrometer.
Kruppa; Gary (San Francisco, CA), Schoeniger; Joseph S. (Oakland, CA), Young; Malin M. (Livermore, CA)
Sandia National Laboratories (Livermore, CA)
10/ 437,268
May 12, 2003
GOVERNMENT RIGHTS This invention was made with government support under Contract No. DE-AC04-4AL85000 by the United States Department of Energy. The government has certain rights in the invention.