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Arrays of probes for positional sequencing by hybridization

United States Patent

January 15, 2008
View the Complete Patent at the US Patent & Trademark Office
This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.
Cantor; Charles R. (Boston, MA), Prezetakiewiczr; Marek (East Boston, MA), Smith; Cassandra L. (Boston, MA), Sano; Takeshi (Waltham, MA)
The Trustees of Boston University (Boston, MA)
09/ 030,571
February 24, 1998
This invention was made with Government support under grant number DE-FG02-93-ER61609 awarded by the Department of Energy. The United States Government may have certain rights in this invention.