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Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants

United States Patent

July 10, 2007
View the Complete Patent at the US Patent & Trademark Office
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Cellulose synthase ("CS"), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl)phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.
Somerville; Chris R. (Portola Valley, CA), Scheible; Wolf (Golm, DE)
U.S. Department of Energy (Washington, DC)
10/ 721,996
November 26, 2003
U.S. GOVERNMENT RIGHTS The United States Government has rights in this invention pursuant to Contract No. DE-FG02-94ER20133 between the U.S. Department of Energy and the Carnegie Institute of Washington.