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Chemical inducible promoter used to obtain transgenic plants with a silent marker and organisms and cells and methods of using same for screening for mutations

United States Patent

June 12, 2007
View the Complete Patent at the US Patent & Trademark Office
Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
Zuo; Jianru (New York, NY), Chua; Nam-Hai (Scarsdale, NY)
The Rockefeller University (New York, NY)
10/ 129,849
November 13, 2000
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH This application was made in part with Government support under DOE Grant #FG02-94ER20142, funded by the Department of Energy.