The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.
GOVERNMENT SPONSORED RESEARCH
This work was supported, in part, by grants from the United States Department of Agriculture, National Research Initiative (95-37308-1843; 98-35504-6177), and United States Department of Energy (DE-FG02-96ER20222). The Government has certain rights in the invention.