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Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change

United States Patent

January 2, 2007
View the Complete Patent at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.
Miles; Robin R. (Danville, CA), Belgrader; Phillip (Severna Park, MD), Fuller; Christopher D. (Oakland, CA)
The Regents of the University of California (Oakland, CA)
11/ 020,731
December 21, 2004
The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.