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High-throughput, dual probe biological assays based on single molecule detection

United States Patent

7,076,092
July 11, 2006
View the Complete Patent at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 .mu.m.times.100 .mu.m.times.100 .mu.m. A device using these methods would have 10.sup.3 10.sup.4 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 .mu.m.sup.3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (.about.10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.
Hollars; Christopher W. (Brentwood, CA), Huser; Thomas R. (Livermore, CA), Lane; Stephen M. (Oakland, CA), Balhorn; Rodney L. (Livermore, CA), Bakajin; Olgica (San Leandro, CA), Darrow; Christopher (Pleasanton, CA), Satcher, Jr.; Joe H. (Patterson, CA)
The United States of America as represented by the United States Department of Energy (Washington, DC), N/A (
10/ 170,876
20060062440
June 12, 2002
The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.