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Cast-to-shape electrokinetic trapping medium

United States Patent

May 30, 2006
View the Complete Patent at the US Patent & Trademark Office
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A three-dimensional microporous polymer network material, or monolith, cast-to-shape in a microchannel. The polymer monolith, produced by a phase separation process, is capable of trapping and retaining charged protein species from a mixture of charged and uncharged species under the influence of an applied electric field. The retained charged protein species are released from the porous polymer monolith by a pressure driven flow in the substantial absence of the electric field. The pressure driven flow is independent of direction and thus neither means to reverse fluid flow nor a multi-directional flow field is required, a single flow through the porous polymer monolith can be employed, in contrast to prior art systems. The monolithic polymer material produced by the invention can function as a chromatographic medium. Moreover, by virtue of its ability to retain charged protein species and quantitatively release the retained species the porous polymer monolith can serve as a means for concentrating charged protein species from, for example, a dilute solution.
Shepodd; Timothy J. (Livermore, CA), Franklin; Elizabeth (Rolla, MO), Prickett; Zane T. (Golden, CO), Artau; Alexander (Pleasanton, CA)
Sandia National Laboratories (Livermore, CA)
10/ 841,393
May 7, 2004
STATEMENT OF GOVERNMENT INTEREST This invention was made with Government support under contract no. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention.