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Method for metabolizing carbazole in petroleum

United States Patent

September 13, 2005
View the Complete Patent at the US Patent & Trademark Office
A method for selective cleavage of C--N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C--N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C--N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C--N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.
Kayser; Kevin J. (Chesterfield, MI), Kilbane, II; John J. (Woodstock, IL)
Alps Electric Co., Ltd. (Tokyo, JP)
10/ 658,691
September 9, 2003
The U.S. Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of Contract No. DE-AC26-99BC 15219 awarded by the U.S. Department of Energy.